Overview

  • Product name
  • Description
    Rabbit polyclonal to PASK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1273 -1323 of Human PASK (Q96RG2).

  • Positive control
    • HeLa, 293T and Jurkat cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 143 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity
    Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on Thr-1161 and Thr-1165.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP434O051 antibody
    • DKFZp686P2031 antibody
    • hPASK antibody
    • KIAA0135 antibody
    • MGC93882 antibody
    • mKIAA0135 antibody
    • PAS domain containing serine/threonine kinase antibody
    • PAS domain containing serine/threonine protein kinase antibody
    • PAS domain-containing serine/threonine-protein kinase antibody
    • PAS kinase antibody
    • PAS serine/threonine kinase antibody
    • PAS-kinase antibody
    • Pask antibody
    • PASK_HUMAN antibody
    • PASKIN antibody
    • STK 37 antibody
    • STK37 antibody
    see all

Images

  • All lanes : Anti-PASK antibody (ab115164) at 0.4 µg/ml

    Lane 1 : HeLa Cell lysate at 50 µg
    Lane 2 : HeLa Cell lysate at 15 µg
    Lane 3 : 293T Cell lysate at 50 µg
    Lane 4 : Jurkat Cell lysate at 50 µg


    Predicted band size : 143 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation of PASK (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-PASK antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-PASK antibody (ab115164); Lane 3- Immunoprecipitation with IgG control. Anti-PASK antibody (ab115164) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

References

ab115164 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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