Overview

  • Product name
  • Description
    Rabbit polyclonal to PASK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1273 -1323 of Human PASK (Q96RG2).

  • Positive control
    • HeLa, 293T and Jurkat cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 143 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity
    Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on Thr-1161 and Thr-1165.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP434O051 antibody
    • DKFZp686P2031 antibody
    • hPASK antibody
    • KIAA0135 antibody
    • MGC93882 antibody
    • mKIAA0135 antibody
    • PAS domain containing serine/threonine kinase antibody
    • PAS domain containing serine/threonine protein kinase antibody
    • PAS domain-containing serine/threonine-protein kinase antibody
    • PAS kinase antibody
    • PAS serine/threonine kinase antibody
    • PAS-kinase antibody
    • Pask antibody
    • PASK_HUMAN antibody
    • PASKIN antibody
    • STK 37 antibody
    • STK37 antibody
    see all

Anti-PASK antibody images

  • All lanes : Anti-PASK antibody (ab115164) at 0.4 µg/ml

    Lane 1 : HeLa Cell lysate at 50 µg
    Lane 2 : HeLa Cell lysate at 15 µg
    Lane 3 : 293T Cell lysate at 50 µg
    Lane 4 : Jurkat Cell lysate at 50 µg


    Predicted band size : 143 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation of PASK (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-PASK antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-PASK antibody (ab115164); Lane 3- Immunoprecipitation with IgG control. Anti-PASK antibody (ab115164) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

References for Anti-PASK antibody (ab115164)

ab115164 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115164.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up