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Rabbit polyclonal to PASK - C-terminal
Recombinant fragment, corresponding to a region within C terminal amino acids 982-1227 of Human PASK (Uniprot ID: Q96RG2).
- Mouse brain whole cell lysate; HeLa cells; Human NCIN87 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 143 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain.
Autophosphorylated on Thr-1161 and Thr-1165.
Information by UniProt
- DKFZP434O051 antibody
- DKFZp686P2031 antibody
- hPASK antibody
Western blot - Anti-PASK antibody - C-terminal (ab154053)
Anti-PASK antibody - C-terminal (ab154053) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size : 143 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PASK antibody - C-terminal (ab154053)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PASK with ab154053 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PASK antibody - C-terminal (ab154053)
Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue, labeling PASK with ab154053 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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