Overview

  • Product name
    Anti-PASK antibody - C-terminal
    See all PASK primary antibodies
  • Description
    Rabbit polyclonal to PASK - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 982-1227 of Human PASK (Uniprot ID: Q96RG2).

  • Positive control
    • Mouse brain whole cell lysate; HeLa cells; Human NCIN87 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 143 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity
    Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on Thr-1161 and Thr-1165.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP434O051 antibody
    • DKFZp686P2031 antibody
    • hPASK antibody
    • KIAA0135 antibody
    • MGC93882 antibody
    • mKIAA0135 antibody
    • PAS domain containing serine/threonine kinase antibody
    • PAS domain containing serine/threonine protein kinase antibody
    • PAS domain-containing serine/threonine-protein kinase antibody
    • PAS kinase antibody
    • PAS serine/threonine kinase antibody
    • PAS-kinase antibody
    • Pask antibody
    • PASK_HUMAN antibody
    • PASKIN antibody
    • STK 37 antibody
    • STK37 antibody
    see all

Images

  • Anti-PASK antibody - C-terminal (ab154053) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 143 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PASK with ab154053 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue, labeling PASK with ab154053 at 1/500 dilution.

References

ab154053 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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