Overview

  • Product nameAnti-Patched / PTCH1 antibody
    See all Patched / PTCH1 primary antibodies
  • Description
    Rabbit polyclonal to Patched / PTCH1
  • Specificityab53715 detects endogenous levels of total Patched/PTCH1 protein. In WB, in addition to the expected band at 150 kDa an extra band at 75 kDa is often detected. Both bands can be blocked with the immunogen peptide. In IHC, cytoplasmic and membrane staining is frequently observed. These results are similar to what we see with other antibodies against this target.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Patched/ PTCH1 (N terminal). Immunogen is in the range of aa 1-50.
    Database link: Q13635

  • Positive control
    • IHC-P: Human brain tissue WB: Mouse muscle membrane lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab53715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 161 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionActs as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
  • Tissue specificityIn the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.
  • Involvement in diseaseDefects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
    Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
    Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • Sequence similaritiesBelongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • Developmental stageIn the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  • Post-translational
    modifications
    Glycosylation is necessary for SHH binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A230106A15Rik antibody
    • BCNS antibody
    • FLJ26746 antibody
    • FLJ42602 antibody
    • Holoprosencephaly 7 antibody
    • HPE7 antibody
    • mes antibody
    • NBCCS antibody
    • OTTHUMP00000021709 antibody
    • OTTHUMP00000021710 antibody
    • Patched antibody
    • Patched (Drosophila) homolog antibody
    • Patched 1 antibody
    • Patched homolog (Drosophila) antibody
    • Patched homolog 1 (Drosophila) antibody
    • Patched homolog 1 antibody
    • Patched protein homolog 1 antibody
    • Protein patched homolog 1 antibody
    • PTC antibody
    • PTC1 antibody
    • PTC1_HUMAN antibody
    • PTCH antibody
    • PTCH protein +12b antibody
    • PTCH protein +4' antibody
    • PTCH protein -10 antibody
    • PTCH protein antibody
    • ptch1 antibody
    • PTCH1 protein antibody
    • PTCH11 antibody
    • Ptch2 antibody
    see all

Anti-Patched / PTCH1 antibody images

  • All lanes : Anti-Patched / PTCH1 antibody (ab53715) at 1/500 dilution

    Lane 1 : Extracts from mouse muscle cells, minus immunising peptide
    Lane 2 : Extracts from mouse muscle cells, plus immunising peptide


    Predicted band size : 161 kDa
  • ab53715, at a 1/50 dilution, staining Patched / PTCH in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.ab53715, at a 1/50 dilution, staining Patched / PTCH in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

References for Anti-Patched / PTCH1 antibody (ab53715)

This product has been referenced in:
  • Gao Y  et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014). ICC ; Mouse . Read more (PubMed: 24803588) »
  • Im S  et al. Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival. Korean J Pathol 47:116-23 (2013). IHC-P ; Human . Read more (PubMed: 23667370) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (placenta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate and 10 mM citric acid
Permeabilization No
Specification placenta
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
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Submitted Dec 18 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (ATDC5)
Gel Running Conditions Non-reduced Denaturing (10%)
Loading amount 70 µg
Treatment Dicoction for 48 h
Specification ATDC5
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Dec 18 2015

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Human Cell lysate - whole cell (fibroblasts)
Specification fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Mar 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MKN45)
Specification MKN45
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (7% PAGE)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (chondrocytes)
Loading amount 30 µg
Specification chondrocytes
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
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Submitted Dec 10 2012

Thank you for your enquiry and your interest in our products. The production and the testing conditions of these two antibodies (ab53715 and ab55629) are different.

It is important to mention that ab55629 has only been tested in WB against t...

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Thank you for your enquiry. Both the antibodies ab27529 and ab53715 have been tested in mouse tissue in IHC-P so should be suitable for your use. The datasheet for ab27529 indeed mentions an incubation time of 10 minutes, as this is the originator...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"