Overview

  • Product name
  • Description
    Rabbit polyclonal to PATJ
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 540-589 (EVMVATLDTQ IADDAELQKY SKLLPIHTLR LGVEVDSFDG HHYISSIVSG) of Human PATJ (NP_795352).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 196 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    PATJ contains multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. PATJ localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.
  • Cellular localization
    Membrane. Cell junction; tight junction. Apical cell membrane. Note: Localized in the paranodal region of myelinating Schwann cells. Membrane-associated. Localizes to tight junctions in epithelial cells. Also found at the apical plasma membrane.
  • Database links
  • Alternative names
    • Channel interacting PDZ domain protein antibody
    • Cipp antibody
    • FLJ26982 antibody
    • hINADL antibody
    • Inactivation no after potential D like protein antibody
    • INAD like antibody
    • InaD like Drosophila antibody
    • INAD like protein antibody
    • INADL antibody
    • INADL protein antibody
    • PALS 1 associated tight junction protein antibody
    • PALS1 associated tight junction protein antibody
    • PATJ antibody
    • PDZ domain protein antibody
    • PDZ domain protein Drosophila inaD like antibody
    • Post synaptic density 95 / discs large / zonula occludens 1 domain protein antibody
    • Protein associated to tight junctions antibody
    see all

Images

  • Anti-PATJ antibody (ab84932) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 196 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

References

ab84932 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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