Overview

  • Product name
  • Description
    Rabbit polyclonal to PATL1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 360-457 (QHRRLLHQRQ QQNRSQHRNL NGAGDRGSHR SSHQDHLRKD PYANLMLQRE KDWVSKIQMM QLQSTDPYLD DFYYQNYFEK LEKLSAAEEI QGDGPKKE) of Human PATL1.

  • Positive control
    • Human colon tissue and A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml. Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the PAT1 family.
  • Domain
    The region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
    The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPat1b antibody
    • OK/KNS-cl.5 antibody
    • PAT1-like protein 1 antibody
    • Pat1b antibody
    • PATL1 antibody
    • PATL1_HUMAN antibody
    • protein associated with topoisomerase II homolog 1 antibody
    • Protein PAT1 homolog 1 antibody
    • Protein PAT1 homolog b antibody
    see all

Images

  • ab122526, at a 1/60 dilution, staining PATL1 in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab122526, at 4 µg/ml, staining PATL1 (green) in PFA-fixed, Triton-permeabilized Human A431 cells by Immunofluorescence.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

ab122526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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