Overview

  • Product nameAnti-PATL1 antibody
    See all PATL1 primary antibodies
  • Description
    Rabbit polyclonal to PATL1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PATL1 (NP_689929.2).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab124257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionRNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the PAT1 family.
  • DomainThe region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
    The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
  • Cellular localizationCytoplasm > P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPat1b antibody
    • OK/KNS-cl.5 antibody
    • PAT1-like protein 1 antibody
    • Pat1b antibody
    • PATL1 antibody
    • PATL1_HUMAN antibody
    • protein associated with topoisomerase II homolog 1 antibody
    • Protein PAT1 homolog 1 antibody
    • Protein PAT1 homolog b antibody
    see all

Anti-PATL1 antibody images

  • All lanes : Anti-PATL1 antibody (ab124257) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 1 kDa


    Exposure time : 3 minutes
  • ab124257 at 1 µg/ml staining PATL1 in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-PATL1 antibody (ab124257)

ab124257 has not yet been referenced specifically in any publications.

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