Overview

  • Product name
  • Description
    Rabbit polyclonal to PATZ1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-154 of Human PATZ1 (Q9HBE1).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58, 69, 74 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Transcriptional repressor.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 BTB (POZ) domain.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8430401L15Rik antibody
    • AI447519 antibody
    • and zinc finger-containing protein 1 antibody
    • AT hook- antibody
    • BTB POZ domain zinc finger transcription factor antibody
    • BTB/POZ domain zinc finger transcription factor antibody
    • dJ400N23 antibody
    • Mazr antibody
    • PATZ antibody
    • PATZ1 antibody
    • PATZ1_HUMAN antibody
    • POZ (BTB) and AT hook containing zinc finger 1 antibody
    • POZ , AT hook, and zinc finger containing protein 1 antibody
    • POZ- antibody
    • Protein kinase A RI subunit alpha associated protein antibody
    • Protein kinase A RI subunit alpha-associated protein antibody
    • RIAZ antibody
    • Transcription factor MAZR antibody
    • ZBTB19 antibody
    • Zfp278 antibody
    • Zinc finger and BTB domain containing protein 19 antibody
    • Zinc finger and BTB domain-containing protein 19 antibody
    • Zinc finger protein 278 antibody
    • Zinc finger protein 278, isoform CRA_d antibody
    • Zinc finger sarcoma gene protein antibody
    • ZNF278 antibody
    • ZSG antibody
    see all

Images

  • ab126903, at 1/250 dilution, staining PATZ1 in Paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
  • ab126903, at 1/500 dilution, stainning PATZ1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is co-stained with Hoechst 33342.
  • Anti-PATZ1 antibody (ab126903) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 58, 69, 74 kDa

References

ab126903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
600000 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Thymocytes)
Specification
Thymocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Jul 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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