Overview

  • Product name
  • Description
    Rabbit polyclonal to PATZ1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 249-468 of Human PATZ1 (UniProt: Q9HBE1).

  • Positive control
    • HepG2, HCT116 cells; A549 and HeLa whole cell lysates; HeLa cells; Human N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcriptional repressor.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 BTB (POZ) domain.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8430401L15Rik antibody
    • AI447519 antibody
    • and zinc finger-containing protein 1 antibody
    • AT hook- antibody
    • BTB POZ domain zinc finger transcription factor antibody
    • BTB/POZ domain zinc finger transcription factor antibody
    • dJ400N23 antibody
    • Mazr antibody
    • PATZ antibody
    • PATZ1 antibody
    • PATZ1_HUMAN antibody
    • POZ (BTB) and AT hook containing zinc finger 1 antibody
    • POZ , AT hook, and zinc finger containing protein 1 antibody
    • POZ- antibody
    • Protein kinase A RI subunit alpha associated protein antibody
    • Protein kinase A RI subunit alpha-associated protein antibody
    • RIAZ antibody
    • Transcription factor MAZR antibody
    • ZBTB19 antibody
    • Zfp278 antibody
    • Zinc finger and BTB domain containing protein 19 antibody
    • Zinc finger and BTB domain-containing protein 19 antibody
    • Zinc finger protein 278 antibody
    • Zinc finger protein 278, isoform CRA_d antibody
    • Zinc finger sarcoma gene protein antibody
    • ZNF278 antibody
    • ZSG antibody
    see all

Images

  • All lanes : Anti-PATZ1 antibody (ab154025) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PATZ1 with ab154025 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human N87 xenograft tissue labeling PATZ1 with ab154025 at 1/500 dilution.

References

This product has been referenced in:
  • Iesato A  et al. PATZ1 knockdown enhances malignant phenotype in thyroid epithelial follicular cells and thyroid cancer cells. Oncotarget 8:82754-82772 (2017). Read more (PubMed: 29137300) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
150000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293T cells)
Specification
HEK293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Nov 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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