Overview

  • Product nameAnti-PAX1 antibody
    See all PAX1 primary antibodies
  • Description
    Rabbit polyclonal to PAX1
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human PAX1.

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat thymus tissue lysates as well as the following whole cell lysates: Jurkat; Raji; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab95227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

Anti-PAX1 antibody images

  • ab95227 staining PAX1 in mouse E11.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 2% paraformaldehyde in PBS for 2 hours at 4°C then placed in 30% sucrose overnight at 4°C prior to embedding in OCT using liquid nitrogen. Tissue was blocked with 1X PBS + 1.5% Milk + 1.5% BSA + 0.1% Triton X-100 for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 23°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-PAX1 antibody (ab95227) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 70 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • All lanes : Anti-PAX1 antibody (ab95227) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-PAX1 antibody (ab95227)

ab95227 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Sample Mouse Tissue sections (E11.5 embryos)
Specification E11.5 embryos
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"