Overview

  • Product name
    Anti-Pax2 antibody
    See all Pax2 primary antibodies
  • Description
    Rabbit polyclonal to Pax2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Zebrafish
  • Immunogen

    A region within synthetic peptide: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR, corresponding to internal sequence amino acids 180-229 of Human Pax2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • IHC-P: Human kidney. ICC: OVCA429 cells (see review) WB: Human liver tissue, OVCAR-5, OVCAR-3, SKOV3, CaoV3 cell lysates (See review)

Properties

Applications

Our Abpromise guarantee covers the use of ab23799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF 1/250. Fix with 3% paraformaldehyde/2% sucrose (see review by R Drapkin).
WB Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 34, 41 kDa (predicted molecular weight: 44 , 42 kDa).Can be blocked with Human Pax2 peptide (ab111670). Dilute antibody in 5% milk in PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • Relevance
    Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localization
    Nuclear
  • Database links
    see all
  • Alternative names
    • FSGS7 antibody
    • Paired box 2 antibody
    • Paired box gene 2 antibody
    • paired box homeotic gene 2 antibody
    • paired box protein 2 antibody
    • Paired box protein Pax 2 antibody
    • Paired box protein Pax-2 antibody
    • Paired box protein Pax2 antibody
    • PAPRS antibody
    • Pax 2 antibody
    see all

Images

  • Western blot - Anti-Pax2 antibody (ab23799)
    Western blot - Anti-Pax2 antibody (ab23799)
    Anti-Pax2 antibody (ab23799) at 2.5 µg/ml + Fetal kidney lysate

    Predicted band size: 44 , 42 kDa
    Observed band size: 34,41 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody (ab23799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody (ab23799)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab23799 at a concentration of 4-8µg/ml. The arrows indicate the positively labelled cells, epithelial cells of the renal tube.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody (ab23799)
    Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody (ab23799)This image was taken from an Abreview submitted on January 5, 2006 from Ron Drapkin. We do not have any further information relating to this image.
    The human ovarian carcinoma cell line OVCA429 was used with ab23799 in ICC/IF. The cells were fixed in paraformaldehyde. Blocking was in 5% serum for 1 hour. The primary antibody was incubated at a dilution of 1/250 for one hour. Immunofluorescence (ICC/IF) only worked with 3% paraformaldehyde/2% sucrose fixation. When 70% methanol/30% acetone fixation was tried non-specific cytoplasmic staining was seen. In the left hand panel staining with DAPI is shown, and in the right staining with the ab23799 antibody.

References

This product has been referenced in:
  • Seipel AH  et al. Immunohistochemical profile of ductal adenocarcinoma of the prostate. Virchows Arch 465:559-65 (2014). Read more (PubMed: 25059847) »
  • Palmieri C  et al. Expression of steroid receptor coactivator 3 in ovarian epithelial cancer is a poor prognostic factor and a marker for platinum resistance. Br J Cancer 108:2039-44 (2013). IHC-P ; Human . Read more (PubMed: 23652306) »

See all 7 Publications for this product

Publishing research using ab23799? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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16 reviews or Q&A

10
Votes: Highest First

Dear Sir/Madam,

Can the product 'Anti-Pax2 antibody - ChIP Grade (ab23799)' be used for chip in chicken tissue? Thank you in advance.

Best regards

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab23799 Pax2 antibody has never been tested with samples from chicken. All tested and guaranteed species cross-reactivity and application information is stated on...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Pax2 antibody - ChIP Grade

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cell)
Permeabilization
Yes - 0,25% Triton X-100
Specification
differentiated induced pluripotent stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Jul 05 2017

How manytests can be done? What's the working dilution for IHC-P? In english, please.

Thank you for your inquiry.

We recommend to use this antibody ata concntration of 4ug/ml in IHC-P (since the conecntration of ab23799 is 1mg/ml, this is a 1:250 dilution). This is only a recommended starting dilution and may well have to be o...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pax2 antibody - ChIP Grade

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Day 14.5 mouse embryo)
Specification
Day 14.5 mouse embryo
Fixative
10% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - 0.1% Triton
Blocking step
Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Apr 02 2012


Please let me know if these numbers work for you:

DISCOUNT CODE:*********
Expiration date: ********


I am very pleased to hear you would like to accept our offer and test ab23799 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem ...

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The order number you requested is as follows: *******. Please let me
know if you require any other information.

Thank you for your reply.

Unfortunately the number you provided is not an Abcam order/reference number. Did you order the antibody directly from Abcam, or through a sub-dealer?

If you can’t find the original Abcam order number...

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testing discount for ab23799, to test on mouse tissue

Thank you very much for your interest in ab23799.

Aswe talkedabout on the phone ab23799 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you can provide me with your original Abcam order number ...

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Thank you very much for your reply. Since there is no other chip-grade Pax2 antibody available, surely I would like to test it in chicken tissue, probably first with a simple western blot and then try the chip. Is it possible that you let me have a lit...

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Thank you very much for your reply.

I am pleased to hear you would be interested in trying ab23799 in chicken samples. I am sorry that because we have over 70 000 products, it is regrettablynot feasible for us to have samples vials to send o...

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Dear All,
could you please let me konw expiration date of the following Abs you have in stock:
AB23799
AB13611
AB93688
AB70008
AB115341

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage...

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Our customer has responded and would like to take up the offer of the testing discount, see below their information;   ii cat number: ab23799 iii immunofluorescence on cryosections, to see the expression of pax2a protein on proximal tubular ep...

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DISCOUNT CODE: XXXXXXXX Expiration date: 13-01-2012 I am very pleased to hear you would like to accept our offer and test ab23799 in Zebrafish. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pl...

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1-10 of 16 Abreviews or Q&A

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