Overview

  • Product nameAnti-Pax2 antibody
    See all Pax2 primary antibodies
  • Description
    Rabbit polyclonal to Pax2
  • Tested applicationsSuitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Zebrafish
    Predicted to work with: Chicken, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Pax2.

    (Peptide available as ab39108.)

  • Positive control
    • This antibody gives a positive signal in the following lysates: Mouse Fetal lysate - 14 days old tissue lysate Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old Mouse Embryonic Fibroblast 1 Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab38738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-Fr 1/100.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevancePax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Paired box 2 antibody
    • Paired box gene 2 antibody
    • paired box homeotic gene 2 antibody
    • paired box protein 2 antibody
    • Paired box protein Pax 2 antibody
    • Paired box protein Pax-2 antibody
    • Paired box protein Pax2 antibody
    • Pax 2 antibody
    see all

Anti-Pax2 antibody images

  • ICC/IF image of ab38738 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38738 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • IHC-Fr image of Pax2 staining on zebrafish retina sections using ab38738 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab38738 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • All lanes : Anti-Pax2 antibody (ab38738) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Mouse Embryonic Fibroblast 1 Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab38738 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-Pax2 antibody (ab38738) at 1/250 dilution

    Lane 1 : Mouse Fetal lysate - 14 Days old Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Mouse Embryonic Fibroblast 1 Whole Cell Lysate
    Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 25 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Pax2 is not expressed in skeletal muscle.

References for Anti-Pax2 antibody (ab38738)

This product has been referenced in:
  • Treviño LS  et al. Gene expression profiling reveals differentially expressed genes in ovarian cancer of the hen: support for oviductal origin? Horm Cancer 1:177-86 (2010). IHC-P ; Chicken . Read more (PubMed: 21761365) »
  • Hurtado A  et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, IHC-P ; Human . Read more (PubMed: 19005469) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Muller Cell, Retina)
Specification Muller Cell, Retina
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Aug 22 2014

Application Western blot
Sample Human Cell lysate - whole cell (MCF7 and MCF7 Tamoxifen resistant)
Loading amount 25 µg
Specification MCF7 and MCF7 Tamoxifen resistant
Treatment Tamoxifen 1 uM 24 hour
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Permeabilization Yes - Tx-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

P Gutwein

Verified customer

Submitted Mar 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"