Anti-Pax2 antibody - ChIP Grade (ab23799)
- Product nameAnti-Pax2 antibody - ChIP GradeSee all Pax2 primary antibodies ...
- DescriptionRabbit polyclonal to Pax2 - ChIP Grade
- Tested applicationsIHC-P, ICC/IF, WB, ChIP more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Zebrafish
A region within synthetic peptide: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR, corresponding to internal sequence amino acids 180-229 of Human Pax2
- Positive control
- IHC-P: Human kidney. ICC: OVCA429 cells (see review) WB: Human liver tissue, OVCAR-5, OVCAR-3, SKOV3, CaoV3 cell lysates (See review)
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab23799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ICC/IF||ICC/IF: 1/250. Fix with 3% paraformaldehyde/2% sucrose (see review by R Drapkin).|
|WB||WB: Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 34, 41 kDa (predicted molecular weight: 44 , 42 kDa).Can be blocked with Human Pax2 peptide (ab111670). Dilute antibody in 5% milk in PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ChIP||ChIP: Use at an assay dependent dilution.|
- RelevancePax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
- Cellular localizationNuclear
- Paired box 2 antibody
- Paired box gene 2 antibody
- paired box homeotic gene 2 antibody
- paired box protein 2 antibody
- Paired box protein Pax 2 antibody
- Paired box protein Pax-2 antibody
- Paired box protein Pax2 antibody
- Pax 2 antibody
Anti-Pax2 antibody - ChIP Grade images
Anti-Pax2 antibody - ChIP Grade (ab23799) at 2.5 µg/ml + Fetal kidney lysate
Predicted band size : 44 , 42 kDa
Observed band size : 34,41 kDa (why is the actual band size different from the predicted?)
ab23799, at an antibody concentration of 4.0 - 8.0 µg/ml, staining Human PAX2 in paraffin embeded kidney tissue. The arrows indicate the positively labelled cells, epithelial cells of the renal tube.
The human ovarian carcinoma cell line OVCA429 was used with ab23799 in ICC/IF. The cells were fixed in paraformaldehyde. Blocking was in 5% serum for 1 hour. The primary antibody was incubated at a dilution of 1/250 for one hour. Immunofluorescence (ICC/IF) only worked with 3% paraformaldehyde/2% sucrose fixation. When 70% methanol/30% acetone fixation was tried non-specific cytoplasmic staining was seen. In the left hand panel staining with DAPI is shown, and in the right staining with the ab23799 antibody.
Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml (ab23799)
References for Anti-Pax2 antibody - ChIP Grade (ab23799)
This product has been referenced in:
- Palmieri C et al. Expression of steroid receptor coactivator 3 in ovarian epithelial cancer is a poor prognostic factor and a marker for platinum resistance. Br J Cancer 108:2039-44 (2013). IHC-P ; Human . Read more (PubMed: 23652306) »
- Viringipurampeer IA et al. Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development. Hum Mol Genet 21:2357-69 (2012). Read more (PubMed: 22357656) »