Overview

  • Product nameAnti-Pax2 antibody - ChIP GradeSee all Pax2 primary antibodies ...
  • Description
    Rabbit polyclonal to Pax2 - ChIP Grade
  • Tested applicationsIHC-P, ICC/IF, WB, ChIP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Zebrafish
  • Immunogen

    A region within synthetic peptide: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR, corresponding to internal sequence amino acids 180-229 of Human Pax2

  • Positive control
    • IHC-P: Human kidney. ICC: OVCA429 cells (see review) WB: Human liver tissue, OVCAR-5, OVCAR-3, SKOV3, CaoV3 cell lysates (See review)

Properties

Applications

Our Abpromise guarantee covers the use of ab23799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF 1/250. Fix with 3% paraformaldehyde/2% sucrose (see review by R Drapkin).
WB Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 34, 41 kDa (predicted molecular weight: 44 , 42 kDa).Can be blocked with Human Pax2 peptide (ab111670). Dilute antibody in 5% milk in PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent dilution.

Target

  • RelevancePax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Paired box 2 antibody
    • Paired box gene 2 antibody
    • paired box homeotic gene 2 antibody
    • paired box protein 2 antibody
    • Paired box protein Pax 2 antibody
    • Paired box protein Pax-2 antibody
    • Paired box protein Pax2 antibody
    • Pax 2 antibody
    see all

Anti-Pax2 antibody - ChIP Grade images

  • Anti-Pax2 antibody - ChIP Grade (ab23799) at 2.5 µg/ml + Fetal kidney lysate

    Predicted band size : 44 , 42 kDa
    Observed band size : 34,41 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab23799 at a concentration of 4-8µg/ml. The arrows indicate the positively labelled cells, epithelial cells of the renal tube.

  • The human ovarian carcinoma cell line OVCA429 was used with ab23799 in ICC/IF. The cells were fixed in paraformaldehyde. Blocking was in 5% serum for 1 hour. The primary antibody was incubated at a dilution of 1/250 for one hour. Immunofluorescence (ICC/IF) only worked with 3% paraformaldehyde/2% sucrose fixation. When 70% methanol/30% acetone fixation was tried non-specific cytoplasmic staining was seen. In the left hand panel staining with DAPI is shown, and in the right staining with the ab23799 antibody.

References for Anti-Pax2 antibody - ChIP Grade (ab23799)

This product has been referenced in:
  • Palmieri C  et al. Expression of steroid receptor coactivator 3 in ovarian epithelial cancer is a poor prognostic factor and a marker for platinum resistance. Br J Cancer 108:2039-44 (2013). IHC-P ; Human . Read more (PubMed: 23652306) »
  • Viringipurampeer IA  et al. Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development. Hum Mol Genet 21:2357-69 (2012). Read more (PubMed: 22357656) »

See all 6 Publications for this product

Product Wall

Thank you for your inquiry.

We recommend to use this antibody ata concntration of 4ug/ml in IHC-P (since the conecntration of ab23799 is 1mg/ml, this is a 1:250 dilution). This is only a recommended starting dilution and may well have to be o...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Day 14.5 mouse embryo)
Specification Day 14.5 mouse embryo
Fixative 10% Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization Yes - 0.1% Triton
Blocking step Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Verified customer

Submitted Apr 02 2012

DISCOUNT CODE:*********
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I am very pleased to hear you would like to accept our offer and test ab23799 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem ...

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Thank you for your reply.

Unfortunately the number you provided is not an Abcam order/reference number. Did you order the antibody directly from Abcam, or through a sub-dealer?

If you can’t find the original Abcam order number...

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Thank you very much for your interest in ab23799.

Aswe talkedabout on the phone ab23799 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you can provide me with your original Abcam order number ...

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Thank you very much for your reply.

I am pleased to hear you would be interested in trying ab23799 in chicken samples. I am sorry that because we have over 70 000 products, it is regrettablynot feasible for us to have samples vials to send o...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab23799 Pax2 antibody has never been tested with samples from chicken. All tested and guaranteed species cross-reactivity and application information is stated on...

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Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage...

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DISCOUNT CODE: XXXXXXXX Expiration date: 13-01-2012 I am very pleased to hear you would like to accept our offer and test ab23799 in Zebrafish. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pl...

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Thank you for your email. We have not tested ab23799 in Zebrafish species so we cannot confirm that the antibody would be suitable. However this does not mean that the antibody cannot be used with Zebrafish cells; this only means we do not have...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"