Anti-PAX4 antibody (ab101721)
Key features and details
- Goat polyclonal to PAX4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PAX4 antibody -
Description
Goat polyclonal to PAX4 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Dog -
Immunogen
Synthetic peptide corresponding to Human PAX4 aa 150-250 (internal sequence). NP_006184.2
Database link: O43316-4 -
Positive control
- Mouse pancreas lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab101721 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity. -
Involvement in disease
Defects in PAX4 are a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2 or maturity-onset diabetes. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Genetic variations in PAX4 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
Defects in PAX4 are a cause of susceptibility to diabetes mellitus ketosis-prone (KPD) [MIM:612227]. KPD is an atypical form of diabetes mellitus characterized by an acute initial presentation with severe hyperglycemia and ketosis, as seen in classic type 1 diabetes, but after initiation of insulin therapy, prolonged remission is often possible with cessation of insulin therapy and maintenance of appropriate metabolic control. Metabolic studies show a markedly blunted insulin secretory response to glucose, partially reversible with the improvement of blood glucose control. Variable levels of insulin resistance are observed, especially in obese patients. Pancreatic beta-cell autoimmunity is a rare finding.
Defects in PAX4 are the cause of maturity-onset diabetes of the young type 9 (MODY9) [MIM:612225]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18506 Mouse
- Entrez Gene: 83630 Rat
- SwissProt: P32115 Mouse
- SwissProt: O88436 Rat
- Unigene: 8026 Mouse
- Unigene: 14531 Rat
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Alternative names
- KPD antibody
- MGC129960 antibody
- MODY9 antibody
see all
Images
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Anti-PAX4 antibody (ab101721) at 0.2 µg/ml + Mouse pancreas lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: ~37 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Datasheets and documents
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Datasheet download
References (2)
ab101721 has been referenced in 2 publications.
- Gao D et al. The Roles of Different Multigene Combinations of Pdx1, Ngn3, Sox9, Pax4, and Nkx2.2 in the Reprogramming of Canine ADSCs Into IPCs. Cell Transplant 31:9636897221081483 (2022). PubMed: 35236160
- Sussman W et al. BMAL1 controls glucose uptake through paired-homeodomain transcription factor 4 in differentiated Caco-2 cells. Am J Physiol Cell Physiol 317:C492-C501 (2019). PubMed: 31216190