Overview

  • Product name
    Anti-PAX5 antibody [1EW]
    See all PAX5 primary antibodies
  • Description
    Mouse monoclonal [1EW] to PAX5
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native PAX5 (purified) (Human)

  • Positive control
    • Human acute lymphoblastic leukemia

Properties

Applications

Our Abpromise guarantee covers the use of ab67005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/20 - 1/40.
    heat induced epitope retrieval for paraffin sections using 10mM citrate buffer (pH6.0) is recommended.

    Note: When utilized in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognised by ab67005. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3%. If these recommendations are not followed, staining intensity will be reduced.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
    • Involvement in disease
      A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
      Leukemia, acute lymphoblastic, 3
    • Sequence similarities
      Contains 1 paired domain.
    • Developmental stage
      Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    Images

    • Human acute lymphoblastic leukemia: immunohistochemical staining for Pax5 with 1/20 ab67005. Note nuclear staining of B cells. Paraffin section.

    References

    ab67005 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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