Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).
Our Abpromise guarantee covers the use of ab15164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42kDa; PAX5