Overview

  • Product nameAnti-PAX5 antibody
    See all PAX5 primary antibodies
  • Description
    Rabbit polyclonal to PAX5
  • Tested applicationsSuitable for: IP, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab15164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
IHC-FoFr
  • Application notesImmunohistology (Formalin/paraffin): 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
    • Involvement in diseaseA chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
      Leukemia, acute lymphoblastic, 3
    • Sequence similaritiesContains 1 paired domain.
    • Developmental stageExpressed at early B-cell differentiation, in the developing CNS and in adult testis.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    Anti-PAX5 antibody images

    • PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 42kDa; PAX5

    • ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

    References for Anti-PAX5 antibody (ab15164)

    This product has been referenced in:
    • Katsushima K  et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . Read more (PubMed: 22736761) »

    See 1 Publication for this product

    Product Wall

    Vielen Dank für Ihren Anruf gestern.

    Ich habe für Sie unsere anti Pax5 Antikörper durchgeschaut und muss Ihnen leider mitteilen, dass auch alle polyklonalen mittels Immunogen-Peptiden hergestellt wurden. Diese werden demnach h&...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (hematopoietic human cell lines)
    Loading amount 10 µg
    Specification hematopoietic human cell lines
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    Submitted Jul 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse pre-B lymphocytes)
    Loading amount 5 µg
    Specification mouse pre-B lymphocytes
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    Submitted Jul 23 2010

    I can confirm that the immunogen sequence is within this amino acid segment and therefore the antibody will recognise your fragment,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"