Overview

  • Product name
  • Description
    Rabbit polyclonal to PAX5
  • Tested applications
    Suitable for: IP, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab15164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
IHC-FoFr
  • Application notes
    Immunohistology (Formalin/paraffin): 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
    • Involvement in disease
      A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
      Leukemia, acute lymphoblastic, 3
    • Sequence similarities
      Contains 1 paired domain.
    • Developmental stage
      Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    Images

    • PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 42kDa; PAX5

    • ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

    References

    This product has been referenced in:
    • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Dog . Read more (PubMed: 28190929) »
    • Perearnau A  et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res 45:5086-5099 (2017). Read more (PubMed: 28158851) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Vielen Dank für Ihren Anruf gestern.

    Ich habe für Sie unsere anti Pax5 Antikörper durchgeschaut und muss Ihnen leider mitteilen, dass auch alle polyklonalen mittels Immunogen-Peptiden hergestellt wurden. Diese werden demnach h&...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hematopoietic human cell lines)
    Loading amount
    10 µg
    Specification
    hematopoietic human cell lines
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    Submitted Jul 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse pre-B lymphocytes)
    Loading amount
    5 µg
    Specification
    mouse pre-B lymphocytes
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    Submitted Jul 23 2010

    I can confirm that the immunogen sequence is within this amino acid segment and therefore the antibody will recognise your fragment,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up