The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/50 - 1/100 (ABC method) for 30-60 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in diseaseA chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6. Leukemia, acute lymphoblastic, 3
Sequence similaritiesContains 1 paired domain.
Developmental stageExpressed at early B-cell differentiation, in the developing CNS and in adult testis.