Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in disease
A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6. Leukemia, acute lymphoblastic, 3
Contains 1 paired domain.
Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
Flow Cytometry analysis of Daudi (human Burkitt's lymphoma) cells labeling PAX5 with unpurified ab92512 at 1/1000 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
References for Anti-PAX5 antibody [EPR3729] (ab92512)
This product has been referenced in:
Deng J et al. Applicability of the methylated CpG sites of paired box 5 (PAX5) promoter for prediction the prognosis of gastric cancer. Oncotarget5:7420-30 (2014).
Read more (PubMed: 25277182) »