- Datasheet
- Specific References (21)
- Protocols
Overview
- Product nameAnti-PAX6 antibody
See all PAX6 primary antibodies - DescriptionRabbit polyclonal to PAX6
- SpecificityThis antibody does not detect bovine PAX6 by Western blot.
- Tested applicationsSuitable for: Flow Cyt, ICC, IP, ICC/IF, WB, IHC-Fr, IHC-Pmore details
- Species reactivityReacts with: Mouse, Rat, Sheep, Cow, Dog, Human, Rhesus monkey
Predicted to work with: Chicken, Xenopus laevis, Non human primates, Zebrafish
- Immunogen
Synthetic peptide corresponding to Mouse PAX6 aa 267-285.
Sequence:REEKLRNQRRQASNTPSHI
(Peptide available asab5895) - Positive control
- Rat whole eye extracts and ovine retinal extracts.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notesPAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters’ anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab5790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC | Use at an assay dependent concentration. | |
| IP | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/50. | |
| WB | 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 50.6 kDa).Can be blocked with PAX6 peptide (ab5895). This antibody can be blocked with ab5895. |
|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 17122106 We recommend optimization of antigen retrieval step using buffers for example Citrate or EDTA with incubations for 5, 10, 15 or 20 minutes in microwave or for 2,3,4 or 5 minutes in pressure cooker. |
Target
- FunctionTranscription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
- Tissue specificityFetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
- Involvement in diseaseDefects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation. - Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. - Developmental stageExpressed in the developing eye and brain.
- Post-translational
modificationsUbiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation. - Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5080 Human
- Entrez Gene: 18508 Mouse
- Entrez Gene: 25509 Rat
- Entrez Gene: 30567 Zebrafish
- Omim: 607108 Human
- SwissProt: P26367 Human
- SwissProt: P63015 Mouse
- SwissProt: P63016 Rat
see all -
Alternative names
- AN 2 antibody
- AN antibody
- AN2 antibody
see all
Anti-PAX6 antibody images
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Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10�M for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1:50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification. -
Western blot - Anti-PAX6 antibody (ab5790)
Predicted band size : 50.6 kDa
Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1:1000 overnight at 4?C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Chemiluminescent detection was performed. -
Immunocytochemistry/ Immunofluorescence - PAX6 antibody - Stem Cell Marker (ab5790)Image from Letzen BS et al, PLoS One. 2010 May 5;5(5):e10480, Fig 1.ab5790 at a 1/20 dilution staining PAX6 (green) in cryosectioned human embryoid bodies by Immunocytochemistry/ Immunofluorescence. -
Immunoprecipitation - Anti-PAX6 antibody (ab5790)Immunoprecipitation of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500�g whole cell lysate with 5�g of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4�C. The immune complexes were captured on 50�l Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4�C then washed in TBST and probed with detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed. -
Immunohistochemistry (Frozen sections) - PAX6 antibody - Stem Cell Marker (ab5790)This image is courtesy of an Abreview submitted by Dr Dean Griffithsab5790 at 1/100 dilution staining mouse E12.5 whole brain tissue sections by IHC-Fr. The tissue section was incubated with the antibody for 14 hours. An Alexa-Fluor ® 555 conjuagetd donkey antibody was used as the secondary. The image shows clear staining in appropriate cell types in the developing CNS and shows expression in radial glia of the neocortex (PMID: 11896398).
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Flow Cytometry - PAX6 antibody - Stem Cell Marker (ab5790)This image is courtesy of an Abreview submitted by Dr Lisa Mohametab5790 staining PAX6 in differentiated mouse ES cells (D3 line) by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 70% methanol. Samples were incubated with primary antibody (1/100 in FACS buffer: 0.2% BSA; 0.1% sodium azide in PBS) for 1 hour. A Phycoerythrin-conjugated goat polyclonal to rabbit IgG (1/500) was used as secondary antibody. Gating strategy: FSC vs SSC on negative control sample.
Purple profile - negative control
Green profile - PAX6 -
Western blot - PAX6 antibody - Stem Cell Marker (ab5790)All lanes : Anti-PAX6 antibody (ab5790)
Lane 1 : rat whole eye extract
Lane 2 : ovine retinal extract
Predicted band size : 50.6 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Shows a Western blot of PAX6 on ovine retinal extract using ab5790. -
Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody - Stem Cell Marker (ab5790)Image courtesy of Tom Hu by Abreview.ab5790 staining PAX6 in murine brain cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, blocked using 5% BSA for 1 hour at room temperature and then incubated with ab5790 at a 1/250 dilution for 4 hours at 4°C. The secondary used was a Cy2 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
References for Anti-PAX6 antibody (ab5790)
This product has been referenced in:
- Yuan X et al. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model. PLoS One 12:e0174074 (2017). IF ; Human . Read more (PubMed: 28441409) »
- Cho SH et al. Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC Ophthalmol 16:193 (2016). Read more (PubMed: 27809828) »
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