Overview

  • Product name
  • Description
    Mouse monoclonal to PAX7
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 411-521 of Human PAX7

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab199010 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab55494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Probable transcription factor. May have a role in myogenesis.
  • Involvement in disease
    Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • Sequence similarities
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Images

  • PAX7 antibody (ab55494) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.
  • PAX7 antibody (ab55494) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

References

This product has been referenced in:
  • Katare R  et al. Clinical-grade human neural stem cells promote reparative neovascularization in mouse models of hindlimb ischemia. Arterioscler Thromb Vasc Biol 34:408-18 (2014). Read more (PubMed: 24202301) »
  • Hamilton DF  et al. Muscle 'regenerative potential' determinesphysical recovery following total knee replacement. Bone Joint Res 2:70-8 (2013). IHC-P ; Human . Read more (PubMed: 23673375) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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