• Product nameAnti-PAX7 antibody [EPR3353]
    See all PAX7 primary antibodies
  • Description
    Rabbit monoclonal [EPR3353] to PAX7
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human PAX7.

  • Positive control
    • 293T, HeLa, Jurkat or HepG2 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab92317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: 1/200.

    WB: 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.

    Is unsuitable for ICC, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbable transcription factor. May have a role in myogenesis.
    • Involvement in diseaseDefects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
    • Sequence similaritiesBelongs to the paired homeobox family.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 paired domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ37460 antibody
      • HUP1 antibody
      • OTTHUMP00000002534 antibody
      • Paired box 7 antibody
      • Paired box gene 7 antibody
      • Paired box homeotic gene 7 antibody
      • Paired box protein Pax-7 antibody
      • Paired domain gene 7 antibody
      • Paired domain gene HuP1 antibody
      • Pax7 antibody
      • PAX7 transcriptional factor antibody
      • PAX7/FKHR fusion gene, included antibody
      • PAX7_HUMAN antibody
      • PAX7B antibody
      • RGD1564360 antibody
      • RMS2 antibody
      see all

    Anti-PAX7 antibody [EPR3353] images

    • All lanes : Anti-PAX7 antibody [EPR3353] (ab92317) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
    • Flow cytometric analysis of HeLa cells using ab92317 (red) at a 1/200 dilution, and rabbit IgG control (green).

    References for Anti-PAX7 antibody [EPR3353] (ab92317)

    This product has been referenced in:
    • Shi H  et al. Improved regenerative myogenesis and muscular dystrophy in mice lacking Mkp5. J Clin Invest 123:2064-77 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23543058) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    We permeabilized the HeLa cells with 1X FACS permeabilizing solution for 10 minutes and blocked with 0.5% BSA in 1X PBS for 30 minutes. We used a 1:200 primary dilution (30 min at room temperature). We use a FITC ...

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    Gracias por tu respuesta.

    Si estas interesada en usar los dos anticuerpos propuestos para citometría, ten en cuenta que el anti-Myf5 no se ha testado en esta técnica, y por tanto no podemos asegurar que baya a funcionar. Por eso...

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    Gracias por contactarnos.

    He llevado a cabo una búsqueda en nuestro catálogo de los anticuerpos solicitados. Contra PAX7 tenemos un anticuerpo, creado en conejo y monoclonal, que se ha testado en ratones por Citometr&i...

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    Thank you for contacting Abcam yesterday.

    The datasheet for the rabbit isotype control used with this antibody in flow cytometry is attached, along with the protocol that was used.

    Please let me know if there is anything else I can ...

    Read More