Overview

  • Product nameAnti-PAX8 antibody
    See all PAX8 primary antibodies
  • Description
    Goat polyclonal to PAX8
  • SpecificityThis antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: PHNSIRSGHGGLN, corresponding to N terminal amino acids 2-14 of Human PAX8.

  • Positive control
    • Human liver and thyroid lysates.
  • General notesGenBank Accession Number - NP_003457; NP_039245; NP_039246; NP_039247; NP_054698

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab13611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human PAX8 peptide (ab23220).

Target

  • FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similaritiesContains 1 paired domain.
  • Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Anti-PAX8 antibody images

  • ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.

  • Anti-PAX8 antibody (ab13611) at 1 µg/ml + Human liver lysate (in RIPA buffer) at 35 µg

    Predicted band size : 48 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection. 



  • Predicted band size : 48 kDa

    Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells).

    PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.

    The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).

    This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.

    Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at

References for Anti-PAX8 antibody (ab13611)

This product has been referenced in:
  • Sicking EM  et al. Subtotal ablation of parietal epithelial cells induces crescent formation. J Am Soc Nephrol 23:629-40 (2012). IHC-P ; Mouse . Read more (PubMed: 22282596) »
  • Borgogni E  et al. Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells. Endocrinology 149:3626-34 (2008). Read more (PubMed: 18372324) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12% acrylamide)
Sample Human Cell lysate - whole cell (renal cancer cell line)
Specification renal cancer cell line
Blocking step Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 30 2014

Thank you for trying our suggestion of using ab97100 instead of the staining kit. You may want to try increasing the dilution to 1:200 to get stronger staining.

Vielen Dank für Ihre rasche Antwort!

Wir haben jetzt die Abreview publiziert und der Code ist aktiv. Vielen Dank für Ihr Feedback!

Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten SieFragen haben oderandere...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Kidney tissue)
Specification Kidney tissue
Fixative Paraformaldehyde
Permeabilization Yes - Triton x100
Blocking step Serum as blocking agent for 4 minute(s) · Concentration: 5.26% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 13 2012

Vielen Dank für Ihre Email und die Abreview.

Ich freue mich zu sehen, dass dieser Antikörper auf Mausgewebe auch funktioniert. Können Sie mir bestätigen, dass Sie gefrorene oder paraffin Schnitte gefärbt haben? Ich ka...

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x

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab13611auf Mausgewebezu testen, annehmen möchten. Entschuldigen Sie bitte, dass ich es nicht mehr geschafft habe, Ihnen den Code gestern zuzusenden.
Wie be...

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Vielen Dank für Ihren Anruf von gestern.

Wie am Telefon diskutiert, schlage ich vor, den Western blot mit einem neuen unverdünnten Aliquot zu wiederholen. Falls dann der Antikörper nicht funktioniert, werden wir das Problem gen...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Da wir leider auch keinen Zugriff zu der Publikation haben, und ein direktes Feedback von Ihnen sehr schätzen würden, kann ich Ihnen zur Zeit ein spe...

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Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage...

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