Overview

  • Product nameAnti-PAX8 antibody
    See all PAX8 primary antibodies
  • Description
    Rabbit polyclonal to PAX8
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH

  • Positive control
    • Induced culture of E.coli (one shot Top10 competent cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab82845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/50000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
    • Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
    • Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
    • Sequence similaritiesContains 1 paired domain.
    • Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • OTTHUMP00000158659 antibody
      • OTTHUMP00000158660 antibody
      • OTTHUMP00000203723 antibody
      • OTTHUMP00000203724 antibody
      • Paired box 8 antibody
      • Paired box gene 8 antibody
      • paired box homeotic gene 8 antibody
      • Paired box protein Pax 8 antibody
      • Paired box protein Pax-8 antibody
      • Paired domain gene 8 antibody
      • PAX 8 antibody
      • PAX8 antibody
      • PAX8_HUMAN antibody
      see all

    Anti-PAX8 antibody images

    • All lanes : Anti-PAX8 antibody (ab82845) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
      Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PAX8 antibody (ab82845)

    ab82845 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab82845.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"