• Product nameAnti-PAX8 antibody
    See all PAX8 primary antibodies
  • Description
    Rabbit polyclonal to PAX8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Xenopus laevis, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).

  • Positive control
    • WB: 293T, H1299, HepG2, MOLT4 or Raji cell lysate; ICC/IF: A549 cells.



Our Abpromise guarantee covers the use of ab97477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similaritiesContains 1 paired domain.
  • Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Anti-PAX8 antibody images

  • Anti-PAX8 antibody (ab97477) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 48 kDa
  • Top: immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab97477 antibody at 1/200 dilution.
    Bottom: PAX8 stain merged with DNA probe stain.

References for Anti-PAX8 antibody (ab97477)

This product has been referenced in:
  • DeJonge RE  et al. Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS One 11:e0162508 (2016). IHC-P . Read more (PubMed: 27607106) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (differentiated induced pluripotent stem cell)
Permeabilization Yes - 0,25% Triton X-100
Specification differentiated induced pluripotent stem cell
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 23 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus tropicalis Cell lysate - whole cell (Whole embryo)
Loading amount 20 µg
Specification Whole embryo
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Florencia del Viso

Verified customer

Submitted May 20 2011