Anti-PAX8 antibody [BC12] (ab124445)

Overview

  • Product name
    Anti-PAX8 antibody [BC12]
    See all PAX8 primary antibodies
  • Description
    Mouse monoclonal [BC12] to PAX8
  • Specificity
    ab124445 does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to PAX8.

  • Positive control
    • Human normal kidney, renal cell or serous ovarian carcinoma tissues.
  • General notes
    Make dilutions in Renoir Red diluent.

Properties

Applications

Our Abpromise guarantee covers the use of ab124445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • ab124445 at 1/100 dilution staining PAX8 in formalin-fixed paraffin-embedded Human Renal cell carcinoma tissue by Immunohistochemistry.

References

ab124445 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (786-0, a renal cell carcinoma cell line)
Loading amount
50 µg
Specification
786-0, a renal cell carcinoma cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2013

1) How was antibody specificity tested?

-We have done ELISA, Western Blot, and IHC for the specificity tests.


2) Do you have any information regarding cross-reactivity of this Pax8 antibody with other Pax-Proteins, especially ...

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Thank you for your patience.

The lab sent me the following information this afternoon:

The ab124445was raised by immunizing full length of human PAX8. The epitope maps to an internal sequence. There is no cross-reactivity with PAX2...

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Thank you for contacting us.

The immunogen isthe recombinantfull-length human PAX8 protein. The exact epitope has not been mapped, therefore we do not know where the antibody would bind in this region.

As for the specificity and cr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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