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Recombinant C-terminal fragment corresponding to Human PAX8
IHC-Fr and IHC-P are not batch-tested and therefore variability between batches might be seen for these applications.
Our Abpromise guarantee covers the use of ab53490 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa.|
|IP||Use at an assay dependent concentration.
|ICC||Use a concentration of 2 - 100 µg/ml.|
ab53490 staining PAX8 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/20 in 10% goat serum in PBS) for 1 hour at 24°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)