Overview

  • Product nameAnti-PAX9 antibody
    See all PAX9 primary antibodies
  • Description
    Rabbit polyclonal to PAX9
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PAX9.

    (Peptide available as ab109893.)

  • Positive control
    • This antibody gave a positive signal in whole cell lysates: U2OS; Jurkat; SAOS-2.

Properties

Applications

Our Abpromise guarantee covers the use of ab95223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • FunctionTranscription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.
  • Involvement in diseaseDefects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).
  • Sequence similaritiesContains 1 paired domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Paired box 9 antibody
    • Paired box gene 9 antibody
    • Paired box homeotic gene 9 antibody
    • Paired box protein 9 antibody
    • Paired box protein Pax 9 antibody
    • Paired box protein Pax-9 antibody
    • Paired box protein Pax9 antibody
    • Paired domain gene 9 antibody
    • PAX 9 antibody
    • PAX9 antibody
    • PAX9_HUMAN antibody
    • STHAG3 antibody
    see all

Anti-PAX9 antibody images

  • All lanes : Anti-PAX9 antibody (ab95223) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 17 kDa,32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-PAX9 antibody (ab95223)

ab95223 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E10.5 mouse embryo sections)
Specification E10.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v Triton X-100 (Sigma)
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Aug 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (mesenchyme)
Loading amount 5 µg
Specification mesenchyme
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
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Verified customer

Submitted Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"