Use at an assay dependent concentration. PubMed: 22629443
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family. Contains 4 LIM zinc-binding domains.
Post-translational modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 68 kDa Observed band size : 68 kDa Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Paxillin antibody (ab2264)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab2264 staining human and mouse pancreas and rat testis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
Immunocytochemistry/ Immunofluorescence - Paxillin antibody (ab2264)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab2264 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.
Immunohistochemistry (Frozen sections) - Anti-Paxillin antibody (ab2264)Image from Phillips HM et al., PLoS One. 2012;7(5):e37685. Epub 2012 May 21. Fig 8.; doi:10.1371/journal.pone.0037685; May 21, 2012, PLoS ONE 7(5): e37685.
Immunohistochemical analysis of frozen sections of frontonasal processes from wild-type (control) or RockDN;Wnt1-cre mutant (RockDN) mouse embyros; Paxillin was stained with ab2264.
References for Anti-Paxillin antibody (ab2264)
This product has been referenced in:
Li A et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics12:203-25 (2012).
Read more (PubMed: 22253135) »
Phillips HM et al. Neural crest cell survival is dependent on Rho kinase and is required for development of the mid face in mouse embryos. PLoS One7:e37685 (2012).
Read more (PubMed: 22629443) »