Overview

  • Product name
    Anti-Paxillin antibody [E228]
    See all Paxillin primary antibodies
  • Description
    Rabbit monoclonal [E228] to Paxillin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Paxillin aa 1-100 (phospho Y31). The exact sequence is proprietary.
    Database link: P49023
    (Peptide available as ab179557)

  • Positive control
    • WB: NIH 3T3 cell lysate. ICC/IF: HeLa cells. IHC-P: Human colon carcinoma.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 64 kDa.
ICC/IF 1/250.
IHC-P 1/100 - 1/250.
IP 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similarities
      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Paxillin with ab32115 at 1/100 (1 μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/ml) was used as the secondary antibody. The cells were counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200, 2.5 μg/ml. Nuclei counterstained with DAPI (blue). 

      Confocal image showing membranous staining on HeLa cells.

    • All lanes : Anti-Paxillin antibody [E228] (ab32115) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HEK-293 (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Immunofluorescence analysis of HeLa cells, staining Paxillin with ab32115.

      Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.

    • All lanes : Anti-Paxillin antibody [E228] (ab32115) at 1/10000 dilution

      Lane 1 : untreated NIH 3T3 cell lysate
      Lane 2 : PDGF treated NIH 3T3 cell lysate

      Predicted band size: 64 kDa
      Observed band size: 64 kDa

    • Direct ELISA antigen dose-response curve using ab32115 at 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    • Dot blot analysis of Paxillin (pY31) peptide (Lane 1) and Paxillin non-phospho peptide (Lane 2) labelling Paxillin with ab32115 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunohistochemical analysis of Paxillin expression in paraffin-embedded human colon carcinoma using 1/100 ab32115.

    References

    This product has been referenced in:
    • Tsai CS  et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . Read more (PubMed: 27910925) »
    • Sausgruber N  et al. Tyrosine phosphatase SHP2 increases cell motility in triple-negative breast cancer through the activation of SRC-family kinases. Oncogene N/A:N/A (2014). WB . Read more (PubMed: 24931162) »

    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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