Overview

  • Product name
    Anti-Paxillin (phospho S126) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho S126)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.

  • Positive control
    • RAW 264.7 cells treated with LPS.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA(IgG, protease free). pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa.

Target

Images

  • All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution

    Lane 1 : Lysate from untreated RAW 264.7 cells
    Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
    Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
    Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
    Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen

    Secondary
    Goat F(ab)2 anti-rabbit IgG HRP conjugate.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Boeckeler K  et al. Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration. J Cell Sci 123:2725-32 (2010). WB ; Rat . Read more (PubMed: 20647370) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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