Anti-Paxillin (phospho S83) antibody (ab31833)

Overview

  • Product name
    Anti-Paxillin (phospho S83) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho S83)
  • Specificity
    ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Mouse) conjugated to KLH corresponding to amino acid residues around serine 83 of paxillin.

  • Positive control
    • Calyculin A treated mouse macrophages.

Properties

Applications

Our Abpromise guarantee covers the use of ab31833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000 (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature). Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similarities
      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Images



    • Predicted band size : 68 kDa

    References

    ab31833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. This antibody has yet to be tested in human or monkey tissue. The conserved site in human paxillin (Ser-83) has high homology with the mouse paxillin (Ser-83). There is only 2 amino acid differences, however there ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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