Anti-Paxillin (phospho S83) antibody (ab31833)

Overview

  • Product name
    Anti-Paxillin (phospho S83) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho S83)
  • Specificity
    ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Mouse) conjugated to KLH corresponding to amino acid residues around serine 83 of paxillin.

  • Positive control
    • Calyculin A treated mouse macrophages.

Properties

Applications

Our Abpromise guarantee covers the use of ab31833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000 (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature). Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similarities
      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Anti-Paxillin (phospho S83) antibody images



    • Predicted band size : 68 kDa

    References for Anti-Paxillin (phospho S83) antibody (ab31833)

    ab31833 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. This antibody has yet to be tested in human or monkey tissue. The conserved site in human paxillin (Ser-83) has high homology with the mouse paxillin (Ser-83). There is only 2 amino acid differences, however there ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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