Anti-Paxillin (phospho Y118) antibody (ab38466)

Overview

  • Product nameAnti-Paxillin (phospho Y118) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho Y118)
  • SpecificityThis antibody is specific for Paxillin only when phosphorylated at tyrosine 118.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human Paxillin around the phosphorylation site of tyrosine 118 (HVYpSF).

  • Positive control
    • Hela cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab38466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10,000.
    WB: 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similaritiesBelongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Anti-Paxillin (phospho Y118) antibody images

    • All lanes : Anti-Paxillin (phospho Y118) antibody (ab38466) at 1/500 dilution

      Lane 1 : Hela cell extract + EGF (Cells were treated with 200ng/ml EGF for 20 minutes)
      Lane 2 : Hela cell extract

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 64 kDa

    References for Anti-Paxillin (phospho Y118) antibody (ab38466)

    ab38466 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"