Overview

  • Product nameAnti-Paxillin (phospho Y118) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho Y118)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 118.

  • Positive control
    • NMµMG cells transfected with EGFP-tagged paxillin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24811250

Target

  • FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similaritiesBelongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Anti-Paxillin (phospho Y118) antibody images



  • Predicted band size : 68 kDa


    Peptide Competition: Extracts prepared from NMµMG cells transfected with EGFP-tagged paxillin were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.75 µg/mL ab4833 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4833 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

References for Anti-Paxillin (phospho Y118) antibody (ab4833)

This product has been referenced in:
  • Wang H  et al. Inhibition of tumor vasculogenic mimicry and prolongation of host survival in highly aggressive gallbladder cancers by norcantharidin via blocking the ephrin type a receptor 2/focal adhesion kinase/paxillin signaling pathway. PLoS One 9:e96982 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24811250) »
  • Lange K  et al. Combined lysophosphatidic acid/platelet-derived growth factor signaling triggers glioma cell migration in a tenascin-C microenvironment. Cancer Res 68:6942-52 (2008). WB ; Human . Read more (PubMed: 18757408) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"