Overview

  • Product name
    Anti-Paxillin (phospho Y118) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho Y118)
  • Specificity
    ab75740 detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).

  • Positive control
    • Human breast carcinoma tissue. Extracts from HeLa cells treated with EGF.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similarities
    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Images

  • ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution

    Lane 1 : HeLa cell extract, untreated
    Lane 2 : HeLa cell extract treated with EGF


    Predicted band size : 64 kDa
    Observed band size : 64 kDa

References

This product has been referenced in:
  • Adam AP  et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016). Read more (PubMed: 27603666) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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