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Rabbit polyclonal to Paxillin (phospho Y118)
ab75740 detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
Mouse, Rat, Human
A synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-Y
Human breast carcinoma tissue. Extracts from HeLa cells treated with EGF.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
1/50 - 1/100.
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Information by UniProt
Paired box protein Pax 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Paxillin (phospho Y118) antibody (ab75740)
ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
Western blot - Paxillin (phospho Y118) antibody (ab75740)
All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution Lane 1 : HeLa cell extract, untreated Lane 2 : HeLa cell extract treated with EGF Predicted band size : 64 kDa Observed band size : 64 kDa
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