Recombinant Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Paxillin (phospho Y118) antibody [EPR1903]
See all Paxillin primary antibodies -
Description
Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and Jurkat cell lysates treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1903 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
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Notes |
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Dot blot
1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
Target
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Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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Database links
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Entrez Gene: 360820 Rat
- Omim: 602505 Human
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- SwissProt: Q66H76 Rat
- Unigene: 446336 Human
see all -
Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
Images
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All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/BST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109547 has been referenced in 7 publications.
- Yu J et al. Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways. Biochem Pharmacol 195:114864 (2022). PubMed: 34861243
- Wang W et al. Astragaloside IV promotes the angiogenic capacity of adipose-derived mesenchymal stem cells in a hindlimb ischemia model by FAK phosphorylation via CXCR2. Phytomedicine 96:153908 (2022). PubMed: 35026516
- Zhang X et al. Resibufogenin Targets the ATP1A1 Signaling Cascade to Induce G2/M Phase Arrest and Inhibit Invasion in Glioma. Front Pharmacol 13:855626 (2022). PubMed: 35656311
- Zhang L et al. miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling. Mol Ther Nucleic Acids 25:567-577 (2021). PubMed: 34589278
- Ma J et al. miR-423-3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1. J Clin Lab Anal 35:e24044 (2021). PubMed: 34714955
- Liu SC et al. Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling. Nat Commun 9:5105 (2018). PubMed: 30504771
- Kishi T et al. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget 7:72113-72130 (2016). PubMed: 27708220