Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family. Contains 4 LIM zinc-binding domains.
Post-translational modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Western blot - Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size : 65 kDa
References for Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
This product has been referenced in:
Kishi T et al. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget7:72113-72130 (2016).
Read more (PubMed: 27708220) »