Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235)

Overview

  • Product nameAnti-Paxillin (phospho Y181) antibody [EP41(2)Y]
    See all Paxillin primary antibodies
  • Description
    Rabbit monoclonal [EP41(2)Y] to Paxillin (phospho Y181)
  • Specificityab76235 only detects Paxillin phosphorylated on Tyrosine 181.
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.

  • Positive control
    • HeLa cell lysate treated with pervanadate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similaritiesBelongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] images

  • All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 64 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235)

This product has been referenced in:
  • Yanagishita T  et al. Actin-binding protein, Espin: a novel metastatic regulator for melanoma. Mol Cancer Res 12:440-6 (2014). WB ; Mouse . Read more (PubMed: 24337068) »
  • Kim SJ  et al. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). PLoS One 6:e25103 (2011). WB ; Human . Read more (PubMed: 21966428) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"