• Product nameAnti-Paxillin (phospho Y31) antibody [E228]
    See all Paxillin primary antibodies
  • Description
    Rabbit monoclonal [E228] to Paxillin (phospho Y31)
  • Specificityab32115 recognises only Paxillin phosphorylated on Tyrosine 31.
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho peptide corresponding to residues surrounding Tyr31 of human Paxillin

  • Positive control
    • NIH 3T3 cell lysate, HeLa cells, human colon carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 64 kDa.
ICC/IF 1/250.
IHC-P 1/100 - 1/250.
IP 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similaritiesBelongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Anti-Paxillin (phospho Y31) antibody [E228] images

    • All lanes : Anti-Paxillin (phospho Y31) antibody [E228] (ab32115) at 1/10000 dilution

      Lane 1 : untreated NIH 3T3 cell lysate
      Lane 2 : PDGF treated NIH 3T3 cell lysate

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
    • Immunofluorescent analysis of Paxillin (phospho Y31) expression in HeLa cell culture, using 1/250 ab32115.
    • Immunohistochemical analysis of Paxillin (phospho Y31) expression in paraffin-embedded human colon carcinoma using 1/100 ab32115.
    • Immunofluorescence analysis of HeLa cells, staining Paxillin (phospho Y31) with ab32115.

      Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.

    References for Anti-Paxillin (phospho Y31) antibody [E228] (ab32115)

    This product has been referenced in:
    • Sausgruber N  et al. Tyrosine phosphatase SHP2 increases cell motility in triple-negative breast cancer through the activation of SRC-family kinases. Oncogene N/A:N/A (2014). WB . Read more (PubMed: 24931162) »
    • Beheshti Zavareh R  et al. Suppression of cancer progression by MGAT1 shRNA knockdown. PLoS One 7:e43721 (2012). ICC/IF ; Human . Read more (PubMed: 22957033) »

    See all 4 Publications for this product

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