The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000. Predicted molecular weight: 64 kDa.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family. Contains 4 LIM zinc-binding domains.
Post-translational modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Immunohistochemical analysis of Paxillin (phospho Y31) expression in paraffin-embedded human colon carcinoma using 1/100 ab32115.
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody [E228] (ab32115)Image from Beheshti Zavareh R et al., PLoS One. 2012;7(9):e43721. doi: 10.1371/journal.pone.0043721. Epub 2012 Sep 5. Fig 3.; doi:10.1371/journal.pone.0043721; September 5, 2012, PLoS ONE 7(9): e43721.
Immunofluorescence analysis of HeLa cells, staining Paxillin (phospho Y31) with ab32115.
Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.
References for Anti-Paxillin (phospho Y31) antibody [E228] (ab32115)
This product has been referenced in:
Sausgruber N et al. Tyrosine phosphatase SHP2 increases cell motility in triple-negative breast cancer through the activation of SRC-family kinases. OncogeneN/A:N/A (2014).
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