The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/2000.
WB: 1/1000. Detects a band of approximately 72 kDa corresponding to the molecular mass of phosphorylated paxillin on SDS PAGE immunoblots of pervanadate treated A431 cells, but not in A431 control cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family. Contains 4 LIM zinc-binding domains.
Post-translational modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.