Anti-Paxillin (phospho Y31) antibody [M102] (ab24786)

Overview

  • Product nameAnti-Paxillin (phospho Y31) antibody [M102]
    See all Paxillin primary antibodies
  • Description
    Mouse monoclonal [M102] to Paxillin (phospho Y31)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH corresponding to amino acid residues around Tyrosine 31 of human Paxillin.

Properties

Applications

Our Abpromise guarantee covers the use of ab24786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 72 kDa corresponding to the molecular mass of phosphorylated paxillin on SDS PAGE immunoblots of pervanadate treated A431 cells, but not in A431 control cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similaritiesBelongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Anti-Paxillin (phospho Y31) antibody [M102] images

    • All lanes : Anti-Paxillin (phospho Y31) antibody [M102] (ab24786)

      Lane 1 : A431 cells (30 mg/lane) serum starved overnight
      Lane 2 : A431 cells (30 mg/lane) treated with pervanadate (1 mMl) for 30 min.

    References for Anti-Paxillin (phospho Y31) antibody [M102] (ab24786)

    ab24786 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab24786.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"