Anti-PBK/SPK antibody (ab226923)
Key features and details
- Rabbit polyclonal to PBK/SPK
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PBK/SPK antibody
See all PBK/SPK primary antibodies -
Description
Rabbit polyclonal to PBK/SPK -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee -
Immunogen
Recombinant fragment within Human PBK/SPK (internal sequence). The exact sequence is proprietary.
Database link: Q96KB5 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IP: A431 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 36,37 kDa.
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Notes |
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IP
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 36,37 kDa. |
Target
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Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. -
Tissue specificity
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated; in a cell-cycle dependent manner at mitosis. - Information by UniProt
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Database links
- Entrez Gene: 464496 Chimpanzee
- Entrez Gene: 534781 Cow
- Entrez Gene: 55872 Human
- Entrez Gene: 52033 Mouse
- Entrez Gene: 100141310 Pig
- Entrez Gene: 290326 Rat
- Omim: 611210 Human
- SwissProt: Q96KB5 Human
see all -
Alternative names
- Cancer/testis antigen 84 antibody
- CT84 antibody
- Epididymis luminal protein 164 antibody
see all
Images
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All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PBK/SPK (green) using ab226923 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).
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PBK/SPK was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226923. Western blot was performed from the immunoprecipitate using ab226923. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: A431 whole cell extract (Input).
Lane 2: Control IgG IP in A431 whole cell extract.
Lane 3: ab226923 IP in A431 whole cell extract.
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All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226923 has been referenced in 1 publication.
- Mao P et al. PDZ-Binding Kinase-Dependent Transcriptional Regulation of CCNB2 Promotes Tumorigenesis and Radio-Resistance in Glioblastoma. Transl Oncol 13:287-294 (2020). PubMed: 31874375