Recombinant
RabMAb

Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953)

Overview

  • Product name
    Anti-PBK (phospho T9) antibody [EPR2475(N)]
    See all PBK primary antibodies
  • Description
    Rabbit monoclonal [EPR2475(N)] to PBK (phospho T9)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PBK aa 1-100 (phospho T9). The exact sequence is proprietary.
    Database link: Q96KB5

  • Positive control
    • Hela lysate treated with Nocodazole; Human testis and Rat spleen tissues; Hela cells treated with Nocodazole.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab184953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).

Target

  • Function
    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
  • Tissue specificity
    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 84 antibody
    • CT84 antibody
    • Epididymis luminal protein 164 antibody
    • FLJ14385 antibody
    • HEL164 antibody
    • Lymphokine activated killer T cell originated protein kinase antibody
    • Lymphokine-activated killer T-cell-originated protein kinase antibody
    • MAPKK like protein kinase antibody
    • MAPKK-like protein kinase antibody
    • Nori 3 antibody
    • Nori-3 antibody
    • Nori3 antibody
    • PBK antibody
    • PDZ binding kinase antibody
    • PDZ-binding kinase antibody
    • Serine/threonine protein kinase antibody
    • Spermatogenesis related protein kinase antibody
    • Spermatogenesis-related protein kinase antibody
    • SPK antibody
    • T LAK cell originated protein kinase antibody
    • T-LAK cell-originated protein kinase antibody
    • TOPK antibody
    • TOPK_HUMAN antibody
    see all

Images

  • All lanes : Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution

    Lane 1 : Hela lysate treated with Nocodazole
    Lane 2 : Hela lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

  • Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK (phospho T9) peptide (blue) or non phosphopeptide (orange).

References

ab184953 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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