Overview

  • Product nameAnti-PBP antibody
    See all PBP primary antibodies
  • Description
    Rabbit polyclonal to PBP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human PBP.

  • Positive control
    • This antibody gave a positive signal in Mouse Hippocampus, Human Brain and Human Spinal Cord tissue lysates, as well as the following whole cell lysates: HeLa; A549; HepG2; A431; HL60.

Properties

Applications

Our Abpromise guarantee covers the use of ab109101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 210 antibody
    • Epididymis secretory protein Li 34 antibody
    • Epididymis secretory protein Li 96 antibody
    • HCNP antibody
    • HCNPpp antibody
    • HEL 210 antibody
    • HEL S 34 antibody
    • HEL S 96 antibody
    • Hippocampal cholinergic neurostimulating peptide antibody
    • Neuropolypeptide h3 antibody
    • PBP antibody
    • PEBP antibody
    • PEBP 1 antibody
    • PEBP-1 antibody
    • Pebp1 antibody
    • PEBP1_HUMAN antibody
    • Phosphatidylethanolamine binding protein antibody
    • Phosphatidylethanolamine binding protein 1 antibody
    • Prostatic binding protein antibody
    • Prostatic-binding protein antibody
    • R kip antibody
    • Raf kinase inhibitor protein antibody
    • Raf kinase inhibitory protein antibody
    • RKIP antibody
    see all

Anti-PBP antibody images

  • All lanes : Anti-PBP antibody (ab109101) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 18 kDa,34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-PBP antibody (ab109101)

ab109101 has not yet been referenced specifically in any publications.

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