Overview

  • Product name
    Anti-PBP antibody
    See all PBP primary antibodies
  • Description
    Goat polyclonal to PBP
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide: DYVPKLYEQLSGK, corresponding to C terminal amino acids 174-186 of Human PBP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • A549, HepG2, HEK293 and human liver lysate.
  • General notes
    GenBank Accession Number – NP_002558.

Properties

Applications

Our Abpromise guarantee covers the use of ab2634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20-22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human PBP peptide (ab22921).

Target

  • Function
    Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • Sequence similarities
    Belongs to the phosphatidylethanolamine-binding protein family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Epididymis luminal protein 210 antibody
    • Epididymis secretory protein Li 34 antibody
    • Epididymis secretory protein Li 96 antibody
    • HCNP antibody
    • HCNPpp antibody
    • HEL 210 antibody
    • HEL S 34 antibody
    • HEL S 96 antibody
    • Hippocampal cholinergic neurostimulating peptide antibody
    • Neuropolypeptide h3 antibody
    • PBP antibody
    • PEBP antibody
    • PEBP 1 antibody
    • PEBP-1 antibody
    • Pebp1 antibody
    • PEBP1_HUMAN antibody
    • Phosphatidylethanolamine binding protein antibody
    • Phosphatidylethanolamine binding protein 1 antibody
    • Prostatic binding protein antibody
    • Prostatic-binding protein antibody
    • R kip antibody
    • Raf kinase inhibitor protein antibody
    • Raf kinase inhibitory protein antibody
    • RKIP antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBP antibody (ab2634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBP antibody (ab2634)
    Immunohistochemical analysis of Human prostate tissue labeling PBP. Steamed antigen retrieval with citrate buffer pH 6.
  • Western blot - Anti-PBP antibody (ab2634)
    Western blot - Anti-PBP antibody (ab2634)
    Anti-PBP antibody (ab2634) at 1 µg/ml + A549 lysate at 35 µg

    Predicted band size: 22 kDa

  • Western blot - Anti-PBP antibody (ab2634)
    Western blot - Anti-PBP antibody (ab2634)
    ab2634 staining (1µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2634 staining (0.01 µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBP antibody (ab2634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBP antibody (ab2634)
    ab2634 (1.5 µg/ml) staining PBP in paraffin-embedded Human Cortex following steamed antigen retrieval with citrate buffer at pH 6.

References

This product has been referenced in:
  • Chen Y  et al. The expression, induction and pharmacological activity of CYP1A2 are post-transcriptionally regulated by microRNA hsa-miR-132-5p. Biochem Pharmacol 145:178-191 (2017). WB . Read more (PubMed: 28822783) »
  • Fougner SL  et al. Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment. J Clin Endocrinol Metab 93:1211-6 (2008). WB ; Human . Read more (PubMed: 18230656) »

See all 3 Publications for this product

Publishing research using ab2634? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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Western blot abreview for Anti-PBP antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus)
Loading amount
50 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Username

Dr. Martin Maurer

Verified customer

Submitted Oct 31 2006

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No specific 22 KD band but non-specific bands with very high molecuar weight checked out. SAMPLE I have 2 kinds of samples. One is his-p...

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I'm very sorry to hear you are experiencing problems with ab2634. Thank you for taking the time to provide your protocol details and image, those helped me a lot understand your problem. The fact that you detect the His-PBP protein with a His antib...

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I have two questions today in regards to two separate antibodies: Ab2634: On the data sheet it shows that there is a band detected at 22 kDa, could you please inform me whether this was run under reducing or non-reducing conditions for WB? Ab...

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My apologies for the delay, I have just received the following information from the source of those two antibodies: -ab2634 Before loading samples had 5% (v/v) beta-mercaptoethanol was added and were boiled for 3 minutes. -ab16050 This antibody...

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