Overview

  • Product nameAnti-PBP antibody
    See all PBP primary antibodies
  • Description
    Goat polyclonal to PBP
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide: DYVPKLYEQLSGK, corresponding to C terminal amino acids 174-186 of Human PBP.

  • Positive control
    • A549, HepG2, HEK293 and human liver lysate.
  • General notesGenBank Accession Number – NP_002558.

Properties

Applications

Our Abpromise guarantee covers the use of ab2634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20-22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human PBP peptide (ab22921).

Target

  • FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 210 antibody
    • Epididymis secretory protein Li 34 antibody
    • Epididymis secretory protein Li 96 antibody
    • HCNP antibody
    • HCNPpp antibody
    • HEL 210 antibody
    • HEL S 34 antibody
    • HEL S 96 antibody
    • Hippocampal cholinergic neurostimulating peptide antibody
    • Neuropolypeptide h3 antibody
    • PBP antibody
    • PEBP antibody
    • PEBP 1 antibody
    • PEBP-1 antibody
    • Pebp1 antibody
    • PEBP1_HUMAN antibody
    • Phosphatidylethanolamine binding protein antibody
    • Phosphatidylethanolamine binding protein 1 antibody
    • Prostatic binding protein antibody
    • Prostatic-binding protein antibody
    • R kip antibody
    • Raf kinase inhibitor protein antibody
    • Raf kinase inhibitory protein antibody
    • RKIP antibody
    see all

Anti-PBP antibody images



  • Predicted band size : 22 kDa


    ab2634 staining (0.01 µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab2634 (1.5 µg/ml) staining PBP in paraffin-embedded Human Cortex following steamed antigen retrieval with citrate buffer at pH 6.

References for Anti-PBP antibody (ab2634)

This product has been referenced in:
  • Fougner SL  et al. Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment. J Clin Endocrinol Metab 93:1211-6 (2008). WB ; Human . Read more (PubMed: 18230656) »
  • Chen Q  et al. Identification and characterization of PEBP as a calpain substrate. J Neurochem 99:1133-41 (2006). Read more (PubMed: 17018026) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus)
Loading amount 50 µg
Specification Hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Martin Maurer

Verified customer

Submitted Oct 31 2006

I'm very sorry to hear you are experiencing problems with ab2634. Thank you for taking the time to provide your protocol details and image, those helped me a lot understand your problem. The fact that you detect the His-PBP protein with a His antib...

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My apologies for the delay, I have just received the following information from the source of those two antibodies: -ab2634 Before loading samples had 5% (v/v) beta-mercaptoethanol was added and were boiled for 3 minutes. -ab16050 This antibody...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"