- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-PBP antibody
See all PBP primary antibodies - DescriptionGoat polyclonal to PBP
- Tested applicationsSuitable for: IHC-P, WBmore details
- Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog
- Immunogen
Synthetic peptide: DYVPKLYEQLSGK, corresponding to C terminal amino acids 174-186 of Human PBP.
- Positive control
- A549, HepG2, HEK293 and human liver lysate.
- General notesGenBank Accession Number – NP_002558.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... - PurityIgG fraction
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Immunizing Peptide (Blocking)
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab2634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20-22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human PBP peptide (ab22921). |
Target
- FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. - Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
- Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 431786 Cow
- Entrez Gene: 477501 Dog
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P13696 Cow
- SwissProt: Q3YIX4 Dog
see all -
Alternative names
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
Anti-PBP antibody images
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Western blot - PBP antibody (ab2634)
Predicted band size : 22 kDa
ab2634 staining (0.01 µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBP antibody (ab2634)ab2634 (1.5 µg/ml) staining PBP in paraffin-embedded Human Cortex following steamed antigen retrieval with citrate buffer at pH 6.
References for Anti-PBP antibody (ab2634)
This product has been referenced in:
- Fougner SL et al. Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment. J Clin Endocrinol Metab 93:1211-6 (2008). WB ; Human . Read more (PubMed: 18230656) »
- Chen Q et al. Identification and characterization of PEBP as a calpain substrate. J Neurochem 99:1133-41 (2006). Read more (PubMed: 17018026) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"