Overview

  • Product nameAnti-PBP antibody [EPR2875Y]
    See all PBP primary antibodies
  • Description
    Rabbit monoclonal [EPR2875Y] to PBP
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • HeLa, A549, HepG2 or A431 cell lysate; human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-PBP antibody (HRP) [EPR2875Y] (ab202955)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 22542739
WB 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/10.
ICC/IF 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
      HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
    • Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 210 antibody
      • Epididymis secretory protein Li 34 antibody
      • Epididymis secretory protein Li 96 antibody
      • HCNP antibody
      • HCNPpp antibody
      • HEL 210 antibody
      • HEL S 34 antibody
      • HEL S 96 antibody
      • Hippocampal cholinergic neurostimulating peptide antibody
      • Neuropolypeptide h3 antibody
      • PBP antibody
      • PEBP antibody
      • PEBP 1 antibody
      • PEBP-1 antibody
      • Pebp1 antibody
      • PEBP1_HUMAN antibody
      • Phosphatidylethanolamine binding protein antibody
      • Phosphatidylethanolamine binding protein 1 antibody
      • Prostatic binding protein antibody
      • Prostatic-binding protein antibody
      • R kip antibody
      • Raf kinase inhibitor protein antibody
      • Raf kinase inhibitory protein antibody
      • RKIP antibody
      see all

    Anti-PBP antibody [EPR2875Y] images

    • Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582.
    • All lanes : Anti-PBP antibody [EPR2875Y] (ab76582) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa
    • Anti-PBP antibody [EPR2875Y] (ab76582) at 1/5000 dilution + Recombinant Human PBP protein (ab87892) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Anti-PBP antibody [EPR2875Y] (ab76582)

    This product has been referenced in:
    • Su L  et al. Raf kinase inhibitor protein regulates oxygen-glucose deprivation-induced PC12 cells apoptosis through the NF-?B and ERK pathways. J Clin Biochem Nutr 59:86-92 (2016). WB, IP . Read more (PubMed: 27698534) »
    • Al-Mulla F  et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . Read more (PubMed: 24224123) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (neuronal)
    Specification neuronal
    Fixative Formaldehyde
    Permeabilization Yes - a. PBS + 0.2% Triton x-100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 31 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (NEURONAL)
    Specification NEURONAL
    Fixative Formaldehyde
    Permeabilization Yes - a. PBS + 0.2% Triton x-100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (neuronal)
    Loading amount 50 µg
    Specification neuronal
    Gel Running Conditions Non-reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (neuronal)
    Loading amount 50 µg
    Specification neuronal
    Gel Running Conditions Non-reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"