Recombinant Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RKIP (phospho S153) antibody [EP2845Y]
See all RKIP primary antibodies -
Description
Rabbit monoclonal [EP2845Y] to RKIP (phospho S153) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6 treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate), HeLa treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2845Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
Sequence similarities
Belongs to the phosphatidylethanolamine-binding protein family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5037 Human
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P31044 Rat
- Unigene: 433863 Human
- Unigene: 29745 Rat
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Alternative names
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
Images
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Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate) for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate) for 3 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab75971 has been referenced in 5 publications.
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
- Macrae K et al. Defining the role of DAG, mitochondrial function, and lipid deposition in palmitate-induced proinflammatory signaling and its counter-modulation by palmitoleate. J Lipid Res 54:2366-78 (2013). WB . PubMed: 23833248
- Al-Mulla F et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . PubMed: 24224123
- Deiss K et al. Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem 287:23407-17 (2012). WB . PubMed: 22610096
- Litherland GJ et al. Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction. J Biol Chem 285:22414-25 (2010). PubMed: 20463008