Overview

  • Product nameAnti-PBP (phospho S153) antibody [EP2845Y]
    See all PBP primary antibodies
  • Description
    Rabbit monoclonal [EP2845Y] to PBP (phospho S153)
  • Specificityab75971 detects PBP phosphorylated on serine 153.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding serine 153 of human PBP

  • Positive control
    • HeLa cell lysates treated with calyculin A. Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
      HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
    • Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 210 antibody
      • Epididymis secretory protein Li 34 antibody
      • Epididymis secretory protein Li 96 antibody
      • HCNP antibody
      • HCNPpp antibody
      • HEL 210 antibody
      • HEL S 34 antibody
      • HEL S 96 antibody
      • Hippocampal cholinergic neurostimulating peptide antibody
      • Neuropolypeptide h3 antibody
      • PBP antibody
      • PEBP antibody
      • PEBP 1 antibody
      • PEBP-1 antibody
      • Pebp1 antibody
      • PEBP1_HUMAN antibody
      • Phosphatidylethanolamine binding protein antibody
      • Phosphatidylethanolamine binding protein 1 antibody
      • Prostatic binding protein antibody
      • Prostatic-binding protein antibody
      • R kip antibody
      • Raf kinase inhibitor protein antibody
      • Raf kinase inhibitory protein antibody
      • RKIP antibody
      see all

    Anti-PBP (phospho S153) antibody [EP2845Y] images

    • All lanes : Anti-PBP (phospho S153) antibody [EP2845Y] (ab75971) at 1/2000 dilution

      Lane 1 : HeLa cell lysates un-treated
      Lane 2 : HeLa cell lysates treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa
    • ab75971, at 1/100 dilution, staining PBP in human liver by immunohistochemistry using paraffin-embedded tissue.

    References for Anti-PBP (phospho S153) antibody [EP2845Y] (ab75971)

    This product has been referenced in:
    • Macrae K  et al. Defining the role of DAG, mitochondrial function, and lipid deposition in palmitate-induced proinflammatory signaling and its counter-modulation by palmitoleate. J Lipid Res 54:2366-78 (2013). WB . Read more (PubMed: 23833248) »
    • Al-Mulla F  et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . Read more (PubMed: 24224123) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab75971.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"