Overview

  • Product nameAnti-PBR antibody
    See all PBR primary antibodies
  • Description
    Rabbit polyclonal to PBR
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Dog, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PBR.

  • Positive control
    • This antibody gave a positive signal in Mouse Brown Adipose tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab108489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).

Target

  • FunctionResponsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
  • Tissue specificityFound in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
  • Sequence similaritiesBelongs to the TspO/BZRP family.
  • Cellular localizationMitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Benzodiazapine receptor (peripheral) antibody
    • Benzodiazepine peripheral binding site antibody
    • BPBS antibody
    • BZRP antibody
    • DBI antibody
    • IBP antibody
    • Isoquinoline carboxamide-binding protein antibody
    • MBR antibody
    • mDRC antibody
    • Mitochondrial benzodiazepine receptor antibody
    • PBR antibody
    • PBS antibody
    • Peripheral benzodiazepine receptor antibody
    • Peripheral benzodiazepine receptor-related protein antibody
    • Peripheral type benzodiazepine receptor antibody
    • Peripheral-type benzodiazepine receptor antibody
    • pk18 antibody
    • PKBS antibody
    • PTBR antibody
    • Ptbzr antibody
    • PTBZR02 antibody
    • RATPTBZR02 antibody
    • translocator protein (18kDa) antibody
    • Translocator protein antibody
    • Tspo antibody
    • Tspo1 antibody
    • TSPOA_HUMAN antibody
    see all

Anti-PBR antibody images

  • Anti-PBR antibody (ab108489) at 1 µg/ml + P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-PBR antibody (ab108489)

ab108489 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (H9C2, cardiomyoblast cell line)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification H9C2, cardiomyoblast cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2016

Abreviews
Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - Tween 20
Sample Horse Cell (bone marrow derived mesenchymal stromal cells)
Specification bone marrow derived mesenchymal stromal cells
Gating Strategy 7-AAD negative cells beside debris
Preparation Cell harvesting/tissue preparation method: By Trypsin detached and wasched plastic adherent cells from culture
Sample buffer: PBS+BSA+NaN3+goat serum+Tween20
Username

Ms. Carmen Schröck

Verified customer

Submitted Aug 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"