PBR overexpression 293T lysate (whole cell) (ab94211)

Overview

  • Product namePBR overexpression 293T lysate (whole cell)
  • Description
    PBR overexpression lysate
  • General notesab94211 is a 293T cell transfected lysate in which Human PBR has been transiently over-expressed using a pCMV-PBR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Tissue specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. Similarity: Belongs to the TspO/BZRP family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PBR overexpression 293T lysate (whole cell) images

  • ab94211 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-PBR antibody (ab54895) at 1/500 dilution

    Lane 1 : PBR overexpression 293T lysate (whole cell) (ab94211)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PBR overexpression 293T lysate (whole cell) (ab94211)

ab94211 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"