Overview

  • Product name
  • Description
    Rabbit polyclonal to PBX1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human PBX1.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HL60; K562; MOLT4.

Properties

Applications

Our Abpromise guarantee covers the use of ab97994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.
  • Tissue specificity
    Expressed in all tissues except in cells of the B and T lineage.
  • Involvement in disease
    Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.
  • Sequence similarities
    Belongs to the TALE/PBX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686B09108 antibody
    • Homeo box protein PBX1 antibody
    • Homeo box protein PRL antibody
    • Homeobox protein PBX 1 antibody
    • Homeobox protein PBX1 antibody
    • Homeobox protein PRL antibody
    • MGC126627 antibody
    • PBX 1 antibody
    • Pbx1 antibody
    • PBX1_HUMAN antibody
    • Pre B cell leukemia homeobox 1 antibody
    • Pre B cell leukemia transcription factor 1 antibody
    • Pre-B-cell leukemia transcription factor 1 antibody
    • PRL antibody
    see all

Images

  • All lanes : Anti-PBX1 antibody (ab97994) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
    Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
    Lane 9 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
    Lane 10 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab97994 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97994, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 and Hek392 cells at 5µg/ml.

References

This product has been referenced in:
  • Clarke-Harris R  et al. PGC1a promoter methylation in blood at 5-7 years predicts adiposity from 9 to 14 years (EarlyBird 50). Diabetes 63:2528-37 (2014). Human . Read more (PubMed: 24622795) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cortex)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Cortex
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

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Submitted Oct 31 2017

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