Overview

  • Product nameAnti-PBXIP1 antibody
    See all PBXIP1 primary antibodies
  • Description
    Rabbit polyclonal to PBXIP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide: NQAGGEGAGG ELGISLNMCL LGALVLLGLG VLLFSGGLSE SETGPMEEVE , corresponding to N terminal amino acids 179-228 of Human PBXIP1 (NP_065385)

  • Positive control
    • Lysate from transfected 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevancePBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
  • Cellular localizationCytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus.
  • Database links
  • Alternative names
    • 4732463H20Rik antibody
    • Hematopoietic PBX interacting protein antibody
    • HPIP antibody
    • MGC38361 antibody
    • Pre B cell leukemia homeobox interacting protein 1 antibody
    • RP11-307C12.2 antibody
    see all

Anti-PBXIP1 antibody images

  • Anti-PBXIP1 antibody (ab80504) at 1 µg/ml + lysate from PBXIP1-transfected 293T cells at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PBXIP1 antibody (ab80504)

ab80504 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"