PC12 nuclear extract lysate (forskolin stimulated) (ab14885)

Q&A (1)

Overview

  • Product name
    PC12 nuclear extract lysate (forskolin stimulated)
    See all PC12 lysates
  • Description
    PC12 nuclear extract lysate
  • General notes
    To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay. The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT, 0.37% PBS
    Note: With Protease inhibitor
  • Concentration information loading...
  • Research areas
  • Background
    Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

References

ab14885 has not yet been referenced specifically in any publications.

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Customer reviews and Q&As

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1 Q&A

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Is stimulation with forskolin equivalent to NGF stimulation?

Thank you for your enquiry. Forskolin is a compound that activates the cAMP/PKA-dependent signaling pathway; it activates adenylate cyclase and hence elevates intracellular levels of the second messenger cAMP. cAMP controls metabolism, the cytoskel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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